Fig. 3. Hypoxia increases angiogenic potential of ADMSC-EVs. Representative photomicrographs of HUVEC under different culture conditions for 24 h: EBM (HUVEC cultured in complete EBM medium) (A), DMEM NO FCS (HUVEC cultured in DMEM depleted of FCS) (B), EV CTR (HUVEC cultured in DMEM depleted of FCS, in the presence of EV CTR) (C) and EV HPX (HUVEC cultured in DMEM depleted of FCS, in the presence of EV HPX) (D). Quantification of the number of capillary-like structures/fields observed under each condition (n = 3) (E). Data represent mean ± SEM. (* indicates statistical difference to SHAM group; # indicates statistical difference to IRI group, § indicates statistical difference to IRI+EV CTR group, P<0.05 assessed by one-way ANOVA followed by Tukey's test). Abbreviation: HUVEC, human umbilical vein endothelial cells; EBM, Endothelium Basal Medium; DMEM, Dulbecco's Modified Eagle Medium; FCS, fetal calf serum; EV, extracellular vesicles, CTR, control; HPX, hypoxia.